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ABSTRACT In this comprehensive exploration, we delve into the pivotal role of host plants in shaping the intricate interactions between herbivorous insects and their pathogens. Recent decades have seen a surge in studies that demonstrate that host plants are crucial drivers of the interactions between insects and pathogens, providing novel insights into the direct and indirect interactions that shape tri‐trophic interactions. These studies have built on a wide range of pathogens, from viruses to bacteria, and from protozoans to fungi. We summarise these studies, and discuss the mechanisms of plant‐mediated insect resistance to infection, ranging from the toxicity of plant chemicals to pathogens to enhancement of anti‐pathogen immune responses, and modulation of the insect's microbiome. Although we provide evidence for the roles of all these mechanisms, we also point out that the majority of existing studies are phenomenological, describing patterns without addressing the underlying mechanisms. To further our understanding of these tri‐trophic interactions, we therefore urge researchers to design their studies to enable them specifically to distinguish the mechanisms by which plants affect insect susceptibility to pathogens. 

Abstract Diet profoundly influences the composition of an animal’s microbiome, especially in holometabolous insects, offering a valuable model to explore the impact of diet on gut microbiome dynamics throughout metamorphosis. Here, we use monarch butterflies (Danaus plexippus), specialist herbivores that feed as larvae on many species of chemically well-defined milkweed plants (Asclepias sp.), to investigate the impacts of development and diet on the composition of the gut microbial community. While a few microbial taxa are conserved across life stages of monarchs, the microbiome appears to be highly dynamic throughout the life cycle. Microbial diversity gradually diminishes throughout the larval instars, ultimately reaching its lowest point during the pupal stage and then recovering again in the adult stage. The microbial composition then undergoes a substantial shift upon the transition from pupa to adult, with female adults having significantly different microbial communities than the eggs that they lay, indicating limited evidence for vertical transmission of gut microbiota. While diet did not significantly impact overall microbial composition, our results suggest that fourth instar larvae exhibit higher microbial diversity when consuming milkweed with high concentrations of toxic cardenolide phytochemicals. This study underscores how diet and developmental stage collectively shape the monarch’s gut microbiota.